Applied Biosystems™
Thermal Cyclers (End Point PCR)
 | MiniAmp™ The Applied Biosystems MiniAmp Thermal Cycler is a compact, accurate, and economical thermal cycler designed to fit your lab’s basic thermal cycling needs. The MiniAmp Thermal Cycler has a 5-inch color touchscreen, making it easy to control, and it can also be accessed through Thermo Fisher Connect, giving you the freedom to design and securely upload your methods from any mobile device or desktop computer. Key features and benefits:
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 | MiniAmp™ Plus The Applied Biosystems MiniAmp Plus Thermal Cycler is a compact, accurate, and economical thermal cycler designed to fit your lab’s basic thermal cycling needs. Innovations like a responsive color touch-screen and VeriFlex Blocks temperature-control technology enable simple, accurate optimization. The MiniAmp Plus Thermal Cycler can be accessed through Thermo Fisher Connect, giving you the freedom to design and securely upload your methods from any mobile device or desktop computer. Key features and benefits:
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 | SimpliAmp™ The SimpliAmp™ Thermal Cycler is a small, easy-to-use, and accurate thermal cycler that suits any lab’s needs for everyday PCR. It is crafted with the reliable engineering and performance you’ve come to expect from Applied Biosystem® instruments. It is elegantly designed to deliver quiet cycling, and features an innovative, safe heated lid and a large LCD color touch screen. Key Features and Benefits:
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 | VeritiPro™ The Applied Biosystems VeritiPro Thermal Cycler delivers proven reliability with advanced temperature control technology and connectivity. Take advantage of next-level PCR optimization with the precision offered by VeriFlex block technology. Connect to the cloud-enabled VeritiPro Thermal Cycler remotely. Conveniently design and securely upload your methods, monitor runs, and check instrument availability from any mobile device or desktop computer using Connect, our cloud-based platform. Unique Features of the VeritiPro Thermal Cycler include:
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 | ProFlex™ The ProFlex™ 96-Well PCR System is the latest in our line of Applied Biosystems® thermal cyclers. The ProFlex™ system combines the reliability and performance you’ve come to expect from Applied Biosystems® instruments with the flexible configuration and control features that adapt to your research needs. The ProFlex™ 96-Well PCR System unique features:
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Real-Time PCR Systems
 | QuantStudio 1 The QuantStudio 1 Real-Time PCR System is a basic instrument for new and experienced users looking for a quality product at an affordable price. With a large interactive touchscreen and pre-optimized protocol templates, set up is easy. Run reactions of up to 100 μL and detect differences as small as 1.5-fold in singleplex reactions, with results in approximately 40 minutes. The QuantStudio 1 system is also equipped with Connect, Thermo Fisher Scientific’s cloud-based platform, allowing you to remotely monitor your runs in real time, analyze sophisticated data sets in minutes, securely store data, and share results online with colleagues across institutions and around the world. Features In addition to the common features of all QuantStudio systems, the QuantStudio 1 system offers: Dye compatibility – Three filters calibrated for FAM/SYBR Green/SYTO 9, VIC/HEX/TET/JOE, JUN/ROX/Texas Red dyes Multiplexing capability – Offers the three most commonly used excitation and emission filters: FAM, VIC, and ROX Pre-optimized protocol templates – Pre-optimized protocol templates minimize new user training Connect – Allows you to access, analyze, and share data anytime, anywhere QuantStudio 3 and 5 QuantStudio 3 and 5 real-time PCR systems are factory-calibrated for quick installation and immediate use. These systems detect differences in target quantity as low as 1.5-fold, allow remote monitoring, and feature optional cloud-based Applied Biosystems analysis modules and data sharing. Designed for both new and experienced users, they support 96-well (0.2ml or 0.1ml) or 384-well (QS5 only) consumables. The systems also feature multiplexing capabilities for up to 6 targets and software to enable 21 CFR Part 11 compliance. Features: Adaptable configurations – This compact instrument can be configured as a standalone or with a computer to fit any laboratory needs. Pre-optimized protocol templates – Pre-optimized protocol templates minimize new user training VeriFlex blocks – Applied Biosystems 96-well blocks feature VeriFlex technology with three or six independent temperature zones for precise temperature control and optimization. QuantStudio 3 & 5 qPCR Data Analysis Software – Allows you to remotely monitor your runs, quickly analyze sophisticated data sets, store results in a secure space, and safely share them online More well options – 96-well (0.1-mL block), 96-well (0.2-mL block), and 384-well options Software to assist with 21 CFR Part 11 Compliance – Security, auditing, e-signature package included |
 | QuantStudio Dx Real-Time PCR Instrument Built on a legacy of innovation and excellence in real-time PCR, the QuantStudio™ Dx instrument leverages the best of our portfolio of Applied Biosystems® real-time PCR systems, and refines a platform to specifically meet the needs of the diagnostic laboratory. For In Vitro Diagnostic Use. The QuantStudio™ Dx Real-Time PCR Instrument meets the requirements of the in vitro Diagnostic Medical Devices Directive (98/79/EC). The CE IVD-registered QuantStudio™ Dx Real-Time PCR Instrument is for distribution and use in specific countries only.
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 | QuantStudio 6 and 7 Pro The Applied Biosystems QuantStudio 6 and 7 Pro real-time PCR systems deliver greater flexibility to support a variety of applications and throughput requirements. The systems’ smart features are designed to reduce contamination by allowing you to set up your run hands-free. Schedule and monitor your run from your mobile device, set-up your experiment, analyze data, and share your results from your computer no matter where it is or where you are. The QuantStudio 6 and 7 Pro systems’ 96-well blocks incorporate VeriFlex technology for precise temperature control. These blocks are divided into isolated zones that allow separate cycling conditions within the same run — perfect for optimization. You can also increase your throughput with interchangeable blocks that accommodate 96-well 0.2 ml blocks, 96-well 0.1 ml blocks, 384-well blocks and TaqMan Array Cards (QuantStudio 7 Pro model only). Other unique features of the QuantStudio 7 Pro system include automation compatibility and software features to assist with 21 CFR Part 11 compliance. Many of these features can be added to the QuantStudio 6 Pro system through upgrades to meet changing lab requirements.
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 | QuantStudio 12K Flex The Applied Biosystems QuantStudio 12K Flex Real-Time PCR System can simultaneously run up to four 3,072-reaction QuantStudio 12K Flex OpenArray plates in about four hours. When combined with the QuantStudio 12K Flex OpenArray AccuFill System, the QuantStudio 12K Flex system can produce up to 110,000 data points or more in an 8-hour day with as little as 20 minutes of hands-on time per run. A feature-rich touch screen interface and comprehensive software analysis tools for gene expression, genotyping, and digital PCR increase productivity. Designed for the highest flexibility, the QuantStudio 12K Flex system has five interchangeable block formats including: OpenArray plate, TaqMan Array card, 384-well, and 96-well (0.1 and 0.2 mL). This delivers unmatched versatility for the lab with flexible format requirements. The system also includes laboratory information management system (LIMS) support and features that assist with 21 CFR Part 11 compliance are enabled for high-throughput, validated environments.
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 | QuantStudio 3D Digital Real-Time PCR System The Applied Biosystems QuantStudio 3D Digital PCR System is a simple, affordable platform that uses a sealed-chip technology, providing a streamlined, reliable, and robust method for performing digital PCR. The turn-key chip-based workflow lets you load the chip, seal and proceed to amplification. Expanding the boundaries of traditional real-time PCR the QuantStudio 3D system supports absolute quantification without the use of a standard curve.
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 | 7500 Real-Time PCR System The Applied Biosystems® 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. This system is a sophisticated platform for users who require extended capabilities and maximum versatility. The 3rd generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.
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 | 7500 Fast Real-Time PCR System The Applied Biosystems® 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes.
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 | 7500 Fast Dx Real-Time PCR System The Applied Biosystems® 7500 Fast Dx Real-Time PCR Instrument with SDS Software is a real-time nucleic acid amplification and five-color fluorescence detection system available for in vitro diagnostic use. The 7500 Fast Dx Real-Time PCR Instrument delivers the performance required for high-quality results in a 96-well format.
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Genetic Analyzers
- SeqStudio – Ready. Go. Get results faster with a single-cartridge multi-application genetic analyzer. The new Applied Biosystems SeqStudio Genetic Analyzer is a small-throughput, easy-to-use, and convenient benchtop system that delivers gold-standard technology with just a simple click. It is easily used across a broad range of applications as well as by multiple users. Without complicated setup, you will be ready to go in minutes. The SeqStudio Genetic Analyzer offers data quality, service, and support with a modernized experience at an affordable price. Run diverse Sanger sequencing and fragment analysis applications with the SeqStudio Genetic Analyzer. The features of the SeqStudio Genetic Analyzer make running capillary electrophoresis (CE) experiments easier with minimal hands-on time due to an all-in-one cartridge, facilitate collaboration through Thermo Fisher Cloud–based sharing and applications, and introduce new opportunities to run both sequencing and fragment analysis samples on a single plate.
- 3500 Series Genetic Analyzer – The 3500 Genetic Analyzer is an 8-capillary sequencing instrument specifically designed to support the demanding performance needs of validated and process-controlled environments, while retaining the unsurpassed application versatility that life science researchers expect. The analyzer supports a wide variety of applications, including de novo sequencing and resequencing (mutational profiling), as well as microsatellite analysis, MLPA™, LOH, MLST, AFLP®, and SNP validation or screening.
- Refreshed 3730xL Genetic Analyzer – Scalable. Flexible. Simplified. The Applied Biosystems 3730xl Genetic Analyzer is the highest throughput capillary electrophoresis instrument built for customers who require an economical, automated, and scalable instrument to process large numbers of sequencing or fragment analysis samples. It is now enabled with the Microsoft Windows 10 operating system and Cloud connectivity.
DNA and RNA Purification
 | MagMAX CORE Nucleic Acid Extraction Kits The MagMAX CORE Nucleic Acid Purification Kit is a highly-effective solution for purifying RNA and DNA from an expansive range of veterinary sample types. The universal chemistry is optimized for use on a variety of KingFisher magnetic particle processors and the kit’s modular design enables flexibility to evolve with future testing needs. The MagMAX CORE kit helps to streamline laboratory workflows for downstream real-time PCR, RT-PCR, and sequencing applications. The MagMAX CORE Nucleic Acid Purification Kit offers:
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Real-Time PCR Reagents
 | TaqMan Master Mixes TaqMan™ Master Mixes contain buffer, dNTPs, passive reference dye, thermostable hot-start DNA polymerase, and other components formulated for reliable TaqMan™ Assay-based real-time PCR. Just add your sample and TaqMan Assay, and start your reactions. |
 | TaqPath Master Mixes Enabling confidence and compliance for diagnostic development and testing With superior quality, excellent performance, and regulatory compliance, Applied Biosystems™ TaqPath™ master mixes are designed to deliver confidence and performance for even your most demanding applications. With over 15 years of technology leadership in real-time PCR, Thermo Fisher Scientific is committed to providing clinical laboratories with trusted, versatile, and innovative tools for molecular diagnostic development and testing. TaqPath qPCR master mixes have been validated to deliver exquisite lot-to-lot reproducibility for absolute Ct consistency and dynamic range across a wide variety of assays and applications. These general purpose reagents are manufactured in an ISO 13485 facility and include control measures, change control, document control, and purchasing controls. In addition, each batch is quality control–tested prior to release. TaqPath qPCR master mixes enable superior performance, improved reproducibility, and traceability. |
 | TaqMan Predesigned Assays
Predesigned TaqMan Gene Expression Assay types: TaqMan Gene Expression Assays The largest collection of qPCR gene expression assays. Detect virtually any mRNA transcript in human, mouse, rat, and nearly 30 other model organisms. TaqMan Endogenous Control Assays A convenient collection of TaqMan Gene Expression Assays targeting common endogenous control candidates. Normalization to endogenous control genes is currently the most accurate method to correct for potential biases present in RNA or DNA samples. TaqMan Non-Coding RNA Assays An exclusive set of TaqMan Gene Expression Assays targeting long non-coding RNA transcripts. Stringent assay design criteria produce assays that detect only non-coding transcripts even when coding transcripts are present at the same locus. TaqMan Marker & Reporter Assays A collection of TaqMan Gene Expression Assays enabling detection and quantitation of DNA or RNA sequences encoding common marker and reporter proteins, such as green fluorescent protein (GFP). TaqMan Fusion Assays A specialized set of TaqMan Gene Expression Assays designed to detect rare fusion transcripts that underlie certain cancers. |
 | TaqMan Custom Assays Custom Applied Biosystems TaqMan Expression assays are the custom version of our predesigned real-time PCR 5′ nuclease TaqMan assays for relative quantification of RNA expression. Simply provide us with your target sequence of interest, or submit primer/probe sequences of your own design, and soon you’ll be ready to perform RNA expression studies on any coding or non-coding RNA in any organism. Custom TaqMan® Gene Expression Assays are available for any species, any splice variant, or any novel gene. Simply download our free File Builder Software to format and submit your target sequence. The software easily guides you through the ordering process from selecting the assay size, formatting your target sequence to identify the location of the probe, and submitting your order via e-mail. Compared to do-it-yourself methods, TaqMan® Gene Expression Assays and Custom TaqMan® Gene Expression Assays eliminate weeks or even months of probe and primer design, formulation, and testing. |
 | TaqMan Custom Probes Custom MGB probes – MGB probes offer the best all-around combination of sensitivity, precision, and specificity. MGB technology enables shorter probe designs, resulting in maximal sequence discrimination and target flexibility. Best for detecting up to two targets within the same reaction. Custom TAMRA probes – one of the first TaqMan probes developed, these probes are ideal for maintaining consistency in projects using legacy TAMRA probe designs Custom QSY probes – QSY probes are best-suited for multiplexed detection of more than two targets and pair with proprietary ABY and JUN dyes that are optimized for Applied Biosystems instruments. QSY is ideal for direct conversion of BHQ probe designs |
 | SYBR Green Master Mixes PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up. Coupled with user-supplied primer sets and template, PowerTrack SYBR Green Master Mix is designed to amplify targets for accurate gene expression analysis. Features of PowerTrack SYBR Green Master Mix include:
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PCR Plastics
 | MicroAmp PCR plastics Applied Biosystems MicroAmp plastics consumables offer excellent PCR or qPCR performance in formats developed to meet your experimental needs. All of our plastics consumables are validated with Applied Biosystems instruments for optimal fit and performance. Download the plastics compatibility chart for Applied Biosystems instruments Download PCR Solutions Brochure Our Applied Biosystems EnduraPlate plastic plates offer a solution for experiments that require special handling (i.e., automated and high-throughput workflows) and an even greater degree of durability for use with multi-instrument experiments. We offer 96-well plates, 384-well plates, strip tubes, caps and seals, single tubes, adhesive films, and more. You can use our plastics selection guide to find the right products for your instrument. Even if you don’t currently have an Applied Biosystems instrument in your lab, our selection of MicroAmp plastics has been developed for most applications. Visit thermofisher.com to find the right plastics for your experiment. >> |
 | MicroAmp EnduraPlate Optical Microplates Applied Biosystems MicroAmp EnduraPlate plastic consumables offer excellent PCR or qPCR performance in formats developed to meet your experimental needs. |
Human Identification
 | AutoMate Express Nucleic Acid Extraction System The AutoMate Express Nucleic Acid Extraction System is an easy-to-use benchtop instrument that enables automated nucleic acid extraction. The instrument uses the established PrepFiler Chemistry for high-quality and high-quantity of DNA whether from touch samples or bone tissues. Features of the AutoMate Express Nucleic Acid Extraction System include:
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 | Prep-n-Go Buffer Prep-n-Go™ Buffer (for buccal swabs) is a best-in-class buffer designed to enable high-quality direct PCR amplification of single source samples collected on buccal swabs and amplified using Applied Biosystems® STR chemistries. This product expands direct PCR amplification capabilities to laboratories that use a wide variety of substrates, and is readily adaptable to both manual and automated workflows. No purification, extraction, or quantification is required, thus enabling laboratories to significantly expand their throughput capabilities, while also maximizing sample integrity and data quality. Key Features:
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 | PrepFiler Forensic DNA Extraction Kit The PrepFiler Forensic DNA Extraction Kit uses magnetic particles with an optimized multi-component surface chemistry to deliver superior DNA yield from most forensic sample types, including body fluids, and stains and swabs of body fluids. Whether it’s an automated or manual application, this kit is designed to help improve the overall yield and concentration of DNA recovered from both routine and challenging samples, while enabling removal of PCR inhibitors. This means greater quantities of highly purified DNA for improved downstream genotyping results. This kit is intended for manual use with 1.5 mL tubes. For automated workflows, please see the PrepFiler Automated Forensic DNA Extraction Kit and PrepFiler Express Forensic DNA Extraction Kit.
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 | QuantiFiler DNA Quantification Kits The Quantifiler kits contain all the necessary reagents for the amplification, detection, and quantification of a human-specific DNA target or a human male-specific DNA target.
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 | AmpFLSTR PCR Amplification Kits
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 | GlobalFiler PCR Amplification Kit The GlobalFiler PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, helping to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for casework samples and is also available in a format containing an internal quality control (IQC) system, helping forensic DNA labs worldwide maximize information recovery and improve overall efficiency. For database and single-source samples, please see our GlobalFiler Express PCR Amplification Kit. Key Features:
The GlobalFiler kit is a robust and reliable single-amplification STR multiplex kit optimized for use with the most challenging casework samples. The kit leverages new dyes for the incorporation of additional markers, generating the most discriminating kit available to the forensic community. The kit contains all of the markers included in the major global databases, including 10 mini-STRs, designed to deliver optimized results with even highly degraded samples. Developed specifically for casework applications, the GlobalFiler kit contains a buffer system that delivers greater sensitivity for enhanced performance with inhibited and low-concentration DNA samples. The GlobalFiler kit utilizes the latest advances in oligo synthesis and purification, which provide a clean baseline and a balanced profile, resulting in simplified data analysis. Maintenance of the NGM SElect and Identifiler primer sequences helps ensure maximum concordance with previously typed samples. In addition, multiple gender markers have been added to the multiplex to enable accurate typing of male samples, even highly degraded or Y-negative samples, while increased genotyping accuracy is obtained with the expansion of the allelic ladder allele ranges to include all rare alleles as described in the literature (STRbase). |
 | VeriFiler Plus PCR Amplification Kit The Applied Biosystems VeriFiler Plus PCR Amplification Kit is a highly discriminating, highly sensitive, robust kit optimized for forensic casework. Utilizing our 6-dye chemistry, the VeriFiler Plus kit targets 25 markers: 23 autosomal STRs, including Penta D, Penta E, and two gender discrimination markers. These markers meet all of the requirements CODIS markers as well as the Chinese National autosomal DNA database. The VeriFiler Plus kit was specifically developed to maximize sensitivity to enable maximum information recovery from challenging casework samples including touch, degraded, and inhibited samples, yet can also be run in direct mode for single source reference samples in database workflows. The VeriFiler Plus kit includes all of the same markers as the VeriFiler Express and Huaxia Platinum kits, but in a complementary layout to maximize discrimination for degraded samples. The VeriFiler Plus kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. As with all Applied Biosystems STR kits, the VeriFiler Plus kit is ISO18385 Forensic DNA Grade, giving you piece of mind when using this kit for your casework samples. Key features of the VeriFiler Plus PCR Amplification Kit include:
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 | Precision ID Panels (for NGS) The Applied Biosystems™ Precision ID Identity Panel provides unique genotypes to identify degraded samples for human identification (HID) applications. These genotypes are indispensable for identifying challenging samples as may be the case in low–copy number or low-quality samples, mass disasters, and certain archived samples [1,2,4,5,8]. Precision ID technology enables fast target selection of hundreds of single-nucleotide polymorphisms (SNPs) using multiplex PCR. Thousands of primer pairs can be used in a single tube for target amplification followed by next-generation sequencing (NGS) on the Applied Biosystems™ Precision ID NGS System for HID. |
 | 4N6FLOQSwabs Optimized for collection of reference samples 4N6FLOQSwabs devices utilize proprietary flock technology to maximize DNA collection and elution efficiency. The perpendicular nylon fibers act like a soft brush that facilitates improved collection of cellular material. Unlike traditional fiber-wound swabs, 4N6FLOQSwabs devices have no internal absorbent core to disperse and entrap the specimen—the sample stays close to the surface for faster and more efficient elution. Key features:
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Carrier Screening
 | CarrierMax Kits The CarrierMax FMR1 Reagent Kit is used to detect the number of CGG repeats in the Fragile X Mental Retardation (FMR1) gene. This kit uses a dual PCR system combining full length and triplet primed PCR amplification (TP-PCR), followed by fragment analysis on the Applied Biosystems 3500/3500xL Genetic Analyzer or SeqStudio Genetic Analyzer, to accurately determine up to 200 CGG repeats and detect alleles that are >200 CGG repeats. The CarrierMax SMN1/SMN2 Reagent Kit uses multiplex PCR amplification of genomic DNA and capillary electrophoresis to detect deletions in exon 7 of the Survival of Motor Neuron 1 (SMN1) gene that result in truncated SMN protein. In addition, the kit detects two variants associated with the silent carrier (2+0) haplotype. The results are processed with GeneMapper Software in conjunction with included CarrierMAX Software to consolidate data and simplify carrier classifications in spinal muscular atrophy (SMA) carrier screening research. |
Microsatellite Instability
 | TrueMark MSI Assay The Applied Biosystems TrueMark MSI Assay is a low-input, single-amplification solution for the rapid identification of microsatellite instability (MSI) in formalin-fixed paraffin-embedded (FFPE) samples from multiple tumor tissue types for research applications. The TrueMark MSI Assay leverages an expanded 6-dye set to incorporate additional markers and does not require the use of a tumor-normal match, thereby cutting in half the number of wells required for each sample, as well as reducing the sample handling burden. The assay is compatible with the Applied Biosystems SeqStudio and 3500 Series genetic analyzers. MSI analysis software The associated TrueMark MSI Analysis Software (included at no additional charge) is designed specifically for the TrueMark MSI Assay to enable automated calling of markers and samples, offering greater ease of analysis by reducing the manual QC, analysis, and review required to determine sample status. The automated calling functions without the use of a tumor-normal match, but it is also compatible with tumor-normal pairs. |
Animal Health
 | MagMAX CORE Nucleic Acid Purification The MagMAX CORE Nucleic Acid Purification Kit is a highly-effective solution for purifying RNA and DNA from an expansive range of veterinary sample types. The universal chemistry is optimized for use on a variety of KingFisher magnetic particle processors and the kit’s modular design enables flexibility to evolve with future testing needs. The MagMAX CORE kit helps to streamline laboratory workflows for downstream real-time PCR, RT-PCR, and sequencing applications. The MagMAX CORE Nucleic Acid Purification Kit offers:
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 | AgPath-ID One-Step RT-PCR Reagents
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan® real-time reverse transcription PCR (RT-PCR) strategy.
AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time. Once assembled, results are available in approximately one hour. The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold® polymerase, the preferred hot-start DNA polymerase for specific target amplification. The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization. |
 | VetMAX Xeno IPC The VetMAX™ Xeno™ Internal Positive Control (IPC) – LIZ™ Assay and VetMAX™ Xeno™ Internal Positive Control (IPC) – VIC™ Assay are primer-probe mix that detects the Xeno internal positive control. The resultant Xeno data is used to determine the validity of diagnostic test results. The assay is introduced during the qPCR preparation step and carried through the animal health PCR workflow. VetMAX™ Xeno™ Internal Positive Control (IPC) RNA serves as an internal positive control for the RNA purification process and helps monitor for the presence of PCR inhibitors in animal health molecular detection workflows. This is of particular importance when working with sample types that have a high level of inhibitors such as oral fluids. VetMAX™ Xeno™ Internal Positive Control (IPC) DNA serves as an internal positive control for the DNA purification process and helps monitor for the presence of PCR inhibitors in animal health molecular detection workflows. This is of particular importance when working with sample types that have a high level of inhibitors such as feces. Xeno IPC RNA / DNA is introduced at the nucleic acid isolation/preparation step and carried through the animal health qPCR workflow. |
 | VetMAX PCR Master Mixes The VetMAX™-Plus qPCR Master Mix is designed for multiplex amplification of animal pathogen DNA targets using a rapid qPCR strategy. The kit includes Xeno™ DNA Control, which serves as an internal positive control for DNA purification and qPCR. DNA is amplified using AmpliTaq Gold® DNA Polymerase on a real-time PCR system. The VetMAX™-Plus One-Step RT-PCR Kit is designed for robust amplification of animal pathogen RNA targets using a rapid, single-tube, real-time, reverse transcription PCR (RT-PCR) strategy. The VetMAX™-Plus Multiplex One-Step RT-PCR Kit is designed for multiplex amplification. The kits include Xeno™ RNA Control, which serves as an internal positive control for RNA purification and RT-PCR amplification. RNA is reverse-transcribed into cDNA and the target is amplified using AmpliTaq Gold® DNA Polymerase on a real-time PCR system. For use with VetMAX™ Reagents and VetMAX™ Controls. |
 | GenoTube Livestock Swabs The Applied Biosystems GenoTube Livestock Swab is easy to use and can be handled by even novice users. With its proprietary drying system, it rapidly reduces enzyme activity, preventing the breakdown of nucleic acids and proteins that are essential for many laboratory diagnostic procedures. Once dried, collected samples can be stored without refrigeration for simple and cost-effective transportation. In the lab, the samples can be easily reconstituted and used for numerous analyses. |
SARS-CoV-2 Detection
 | TaqPath COVID-19 CE-IVD RT-PCR Kit
TaqPath™ COVID‑19 CE‑IVD RT‑PCR Kit contains the reagents and controls for a real-time reverse transcription polymerase chain reaction (RT-PCR) test intended for the qualitative detection of nucleic acid from SARS-CoV-2 in upper respiratory specimens (such as nasopharyngeal, oropharyngeal, nasal, and mid‑turbinate swabs, and nasopharyngeal aspirate) and bronchoalveolar lavage (BAL) specimens from individuals suspected of COVID-19. Results are for the identification of SARS-CoV-2 RNA. The SARS-CoV-2 RNA is generally detectable in upper respiratory and bronchoalveolar lavage (BAL) specimens during the acute phase of infection. Positive results are indicative of the presence of SARS-CoV-2 RNA; clinical correlation with patient history and other diagnostic information is necessary to determine patient infection status. Positive results do not rule out bacterial infection or co-infection with other viruses. The agent detected may not be the definite cause of disease. Laboratories may be required to report all positive results to the appropriate Competent Health Authorities. Negative results do not preclude SARS-CoV-2 infection and should not be used as the sole basis for patient management decisions. Negative results must be combined with clinical observations, patient history, and epidemiological information. Testing with the TaqPath™ COVID‑19 CE‑IVD RT‑PCR Kit is intended for use by qualified and trained clinical laboratory personnel specifically instructed and trained in the techniques of real-time PCR and in vitro diagnostic procedures. |
 | TaqPath COVID-19 Combo Kit The Applied Biosystems TaqPath COVID-19 Combo Kit is a fast, highly sensitive multiplex diagnostic solution that contains the assays and controls needed for the real-time PCR detection of RNA from the SARS-CoV-2 virus. Features of the newly developed multiplex diagnostic kit:
The TaqPath COVID-19 Combo Kit consists of both the TaqPath RT-PCR COVID-19 Kit and the TaqPath COVID-19 Control Kit for simplified ordering. The kit can be used by clinical and public health laboratories to quickly evaluate up to 94 patient specimens in under 3 hours. The kit is approved for use with RNA extracted from nasopharyngeal swabs, nasopharyngeal aspirate (nasal aspirate), and bronchoalveolar lavage (BAL) from patients at risk of exposure to the SARS-CoV-2 virus or with signs and symptoms of COVID-19 (see product label for Intended Use Statement and product information). The TaqPath COVID-19 Combo Kit is a single, high-throughput (1,000 reactions) kit and is recommended for use with either the Applied Biosystems 7500 Fast Dx Real-Time PCR System or the 7500 Fast Real-Time PCR System (RUO version) and the associated Applied Biosystems COVID-19 Interpretive Software. The TaqPath COVID-19 Combo Kit includes the following components:
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 | TaqPath COVID-19, Flu A/B, RSV Combo Kit The TaqPath COVID‐19, Flu A/B, RSV Combo Kit contains the reagents and controls for a real-time RT-PCR test for the qualitative detection of nucleic acid from the SARS-CoV-2 virus, the influenza A/B (flu A/B) viruses, and the respiratory syncytial (RSV) virus in nasopharyngeal swabs from individuals showing symptoms of respiratory tract infection. Features of the TaqPath COVID-19, Flu A/B, RSV Combo Kit include:
TaqPath COVID‐19, Flu A/B, RSV Combo Kit includes the following components:
The TaqPath COVID‐19, Flu A/B, RSV Combo Kit is authorized for use on the Applied Biosystems 7500 Fast Real-Time PCR System with nasopharyngeal swab samples only. |
 | TaqCheck SARS-CoV-2 Fast PCR Assay Kit The Applied Biosystems™ TaqCheck™ SARS-CoV-2 Fast PCR Assay is a multiplexed, highly sensitive, fast RT‐PCR assay for the qualitative detection and characterization of SARS-CoV-2 RNA (S and N genes) that compensates for emerging variants and mutations plus a human RNAse P assay to help assess sample adequacy. The SARS-CoV-2 assays target two different viral genomic regions, reducing the risk of failure to detect SARS-CoV-2. The SARS-CoV-2 N and S gene assay targets have undergone bioinformatic selection and analysis to specifically target sequences that are unique to SARS-CoV-2. The assays are designed to work with raw saliva from research samples. The kit is used with the TaqCheck SARS-CoV-2 Control (Cat. No. 956127), TaqCheck SARS-CoV-2 Control Dilution Buffer (Cat. No. A50486), and TaqPath 1-Step RT-qPCR Master Mix, CG (Cat. No A15299 or Cat. No. A15300) (all available separately) to monitor assay-specific amplification. Features of the TaqCheck SARS-CoV-2 Fast PCR Assay include:
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Sanger Sequencing
 | ExoSAP-IT PCR Product Cleanup Kits The Applied Biosystems™ ExoSAP-IT™ PCR Product Cleanup Reagent is widely used for enzymatic PCR cleanup. The one-tube, one-step ExoSAP-IT method has many advantages over using spin columns or magnetic beads for PCR cleanup. With its simple protocol and 100% recovery of both short and long amplicons, ExoSAP-IT reagent enables researchers to conserve limited samples and improve workflow efficiency. In addition to its routine use in Sanger sequencing, ExoSAP-IT reagent is proving beneficial in library preparation workflows across a broad range of NGS applications and platforms for Thermo Fisher Scientific, Illumina, and PacBio, among others. Want to finish PCR product cleanup in just FOUR MINUTES? Go for ExoSAP-IT Express! |
 | BigDye Xterminator Purification Kit The BigDye XTerminator® Purification Kit is a fast, simple purification method for DNA sequencing reactions that removes unincorporated BigDye® terminators and salts. No more dye blobs! Cleanup is complete in under 40 minutes and requires less than 10 minutes of labor.
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 | BigDye Terminator v1.1/v3.1 Cycle Sequencing Kits This BigDye® Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer and for sequencing short PCR product templates with rapid electrophoresis run modules.
The BigDye® Terminator v3.1 Cycle Sequencing Kit’s robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.
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 | BigDye Direct Cycle Sequencing Kit The BigDye® Direct Cycle Sequencing Kit, part of the BigDye® product family, is a complete kit for your PCR and Sequencing needs. This innovative, time-saving kit combines the PCR Purification and Cycle Sequencing Steps, enabling a streamlined protocol and shorter time to results. The kit is available in 24, 100, and 1000-reaction sizes to fit your sequencing throughput needs.
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DNA Analysis Software
 | Sequencing Analysis Software Sequencing Analysis Software uses a base-caller algorithm that performs base calling for pure and mixed base calls. The software analyzes, displays, edits, saves, and prints sample files that are generated from Applied Biosystems DNA analyzers and genetic analyzers. |
 | SeqScape Software SeqScape Software is a resequencing package designed for mutation detection and analysis, SNP discovery and validation, pathogen sub-typing, allele identification, and sequence confirmation. It provides library functions for comparison to a known group of sequences, as well as features to assist with 21 CFR Part 11 compliance (Security, Audit and electronic signature features), which can be important in clinical research labs. |
 | Variant Reporter Software Variant Reporter Software is designed for reference-based and non-reference-based analysis such as mutation detection and analysis, SNP discovery and validation, and sequence confirmation. The robust algorithms will call SNPs, mutations, insertions, deletions, and heterozygous insertions⁄deletions for data generated using Applied Biosystems genetic analyzers. |
 | GeneMapper Software GeneMapper Software is a flexible genotyping software package that provides DNA sizing and quality allele calls for all Applied Biosystems electrophoresis-based genotyping systems. This software specializes in multi-application functionality, including Applied Biosystems amplified fragment length polymorphism (AFLP) analysis, loss of heterozygosity (LOH), microsatellite, and SNP genotyping analysis. In addition, the security and audit features help users meet 21 CFR 11 requirements. |
 | GeneMapper ID-X Software GeneMapper ID-X Software is an automated genotyping solution for forensic casework and databasing data analysis, as well as for paternity data analysis. It supports a multi-user database configuration and provides a set of streamlined data analysis and sample review tools. It also offers a comprehensive quality value system that is designed to be optimized and validated for use in both expert system and traditional data analysis workflows. GeneMapper ID-X Software supports genotyping applications on the Applied Biosystems 310, 3100, 3130, 3130xl, 3500, 3500xl, 3730, and SeqStudio genetic analyzers. The release notes for this version provide information that should allow laboratories to decide whether internal validation testing is necessary for its system to comply with laboratory guidelines or regulations. |
 | Converge Software for NGS Data Analysis Converge Software is an all-in-one, modular, enterprise platform from Thermo Fisher Scientific that provides integrated DNA data management and analysis software for forensic laboratories. Key features and benefits:
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