Cell Culture Media

BenchStable™ Media

Gibco™ BenchStable™ basal media are engineered for flexibility and convenience. The media are formulated with GlutaMAX™ Supplement and are available in widely-used basal media formulations, including: DMEM, DMEM/F-12, MEM, and RPMI 1640. The unique formulation of BenchStable™ media enables room temperature storage of unopened bottles. Complete BenchStable™ media are capable of supporting cellular proliferation and maximum cell densities that are comparable to that of conventional basal media formulations (supplemented with ≥10% FBS). For most cell lines, adaptation is not required to transition from conventional medium to BenchStable™ medium.


Gibco™ Dulbecco’s Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.


Gibco™ Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.

We offer a variety of DMEM/F-12 modifications for a range of cell culture applications.

RPMI 1640

Gibco™ Roswell Park Memorial Institute (RPMI) 1640 Medium has been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle’s Minimal Essential Medium or Dulbecco’s Modified Eagle Medium.

We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications.


Gibco™ Minimum Essential Media (MEM) is patterned after Eagle’s Media and is well suited for the growth of a broad spectrum of mammalian cells.

One of the most commonly used of all cell culture media, MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes.

We offer a variety of MEM modifications for a range of cell culture applications.


Gibco™ GlutaMAX™ Supplement is an alternative to L-glutamine, with increased stability that improves cell health. GlutaMAX Supplement is suitable for both adherent and suspension culture of mammalian cells, with no adaptation required. GlutaMAX Supplement is offered as a 200 mM L-alanyl-L-glutamine dipeptide in 0.85% NaCl. GlutaMAX Supplement is also included in a variety of media formulations. Compared to L-glutamine, GlutaMAX Supplement:

  • Minimizes toxic ammonia build-up
  • Improves cell viability and growth
  • Remains stable across a wide range of temperatures

 Cell Culture Reagents

Balanced Salt Solutions

Trust Gibco™ Balanced Salt Solutions to provide an isotonic buffer system that keeps your cells in the physiological pH range and helps to maintain high viability during short-term incubations.

Available in many formulations and formats, both liquid and powder.  All powdered balanced salt solutions are produced without sodium bicarbonate to increase stability. Powder formats will require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see this protocol for details).

All Gibco Balanced Salt Solutions are manufactured in state-of-the-art cGMP, ISO-certified facilities to ensure the highest quality and consistency for reproducible results every day. Tests include osmolality, pH, stability, the absence of bacterial and fungal contamination, and endotoxin tests.

Trypsin and Cell Dissociation Reagents

Gibco™ trypsin and alternative cell dissociation products are ideal for use with tissues and cell monolayers. Trypsin and TrypLE reagents come in a wide variety of formats to meet the diverse needs of researchers performing adherent cell culture.

Check out all cell dissociation reagents here >>

Distilled Water

Gibco™ distilled water is high-quality water for use as a solvent in the preparation of cell culture media and laboratory reagents. Available in many sizes to meet your needs, this distilled water features:

  • Cell culture quality
  • Proven performance
  • Suitable for use in cell culture and molecular biology applications

Gibco® HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. The addition of 10–25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO2 incubator. HEPES is a good buffering choice for many cell culture systems because it is membrane impermeable, has limited effect on biochemical reactions, is chemically and enzymatically stable, and has very low visible and UV light absorbance.

Cell Freezing Media

Gibco™ Recovery™ Cell Culture Freezing Medium is a complete, ready-to-use freezing medium for cryopreservation of a wide variety of mammalian cells, including CHO-S, CHO-K1, HEK 293, Jurkat, and NIH 3T3. Recovery™ Cell Culture Freezing Medium features:

  • Classic formulation optimized for better performance
  • Suitability for a wide variety of mammalian cells
  • Quality and performance tested

Gibco high-quality selection agents offer unique solutions for your research needs, such as dual selection and rapid stable cell line establishment.

We offer a wide range of antibiotics and antimycotics that can help control or eliminate cell culture contamination.

View the full list of antibiotics and antimycotics >>

Media supplements

Gibco media supplements help ensure reliability and consistency of your cell culture research.

Gibco media supplements include 2-Mercaptoethanol, Insulin, Amino acids, N-2 Supplement, B-27 Supplement, Primary cell supplements, BSA, Sodium pyruvate, Glutamine or GlutaMAX media, Transferrin, and G-5 Supplement.

 Cell Culture Sera

Fetal Bovine Serum (FBS)

Choose the right fetal bovine serum for your specific cell culture needs — from basic research to specialty assays. Whether you need fetal bovine serum with the least viral risk, the lowest endotoxin levels, or sera qualified for specialty applications and assays, Gibco products offer superior value.

Explore FBS products >>

Heat-Inactivated FBS

The Gibco heat-inactivated Fetal bovine serum (FBS) FBS is one of the highest-quality serum products from the brand most trusted by scientists worldwide.

  • Heated for 30 minutes at 56°C with mixing to inactivate complement proteins that are part of the immune response
  • Heat-inactivated serum is especially suited for immunological work
  • Intended for researchers who want to avoid confounding effects, for example in immune response studies using immune cell culture
Specialty FBS

Gibco maintains a large selection of serum products designed for specialty applications and sensitive cell culture, including stem cell research, cancer research, reporter assays, immunoassays, and more.

Choose the specialty serum products for your applications >> 

Other Animal Serum

Gibco serum provides proteins, nutrients, attachment factors, trace elements, growth factors, and hormones, which aids in the growth of the cells.  Although FBS is the most commonly used serum product, many other products are sold as a lower cost alternative. These products include bovine serum, horse serum, newborn calf serum, goat serum, rabbit serum, porcine serum and chicken serum.

View all Other Animal Serum products here >>

Serum-Free Media

Gibco serum-free media promotes superior cell growth and viability

Thermo Fisher Scientific is the world’s largest manufacturer of serum-free cell culture media and a leader in the development of innovative specialty media, including serum-free media options. Serum-free media (SFM) allow researchers to grow a specific cell type or perform a specific application in the absence of serum.

Advantages of using serum-free media include:

  • Easier purification and downstream processing
  • Precise evaluations of cellular function
  • Increased definition
  • More consistent performance
  • Increased growth and/or productivity
  • Better control over physiological responsiveness
  • Enhanced detection of cellular mediators

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