Thermo Scientific™ KingFisher™ Apex is intended for automated transfer and processing of magnetic particles in a microplate format for the purification or isolation of DNA, RNA, proteins, and cells from virtually any source. The Apex moves magnetic beads not liquids through the main steps of sample preparation ‒ bind, wash, elute. This process provides excellent quality, reproducible results, and reduces hands-on time for sample processing.

Reap high yields of quality nucleic acids from a variety of starting materials with the Thermo Scientific™ KingFisher™ Duo Prime purification system. This compact instrument is ideal for laboratories requiring nucleic acid purification, and is easily modified for use in proteomics and cell isolation. It provides excellent reproducibility with minimal hands-on time, and has a small footprint in the lab. New features, including a UV lamp for easy decontamination and optional bar-code reader enhance functionality.

Prepare samples efficiently from a variety of materials with the Thermo Scientific™ KingFisher™ Flex Purification System, which offers highly versatile, automated magnetic-particle processing for DNA/RNA, protein or cell purification from virtually any source. Using revolutionary magnetic particle separation technology, this system provides excellent reproducibility and quality.

Use the Thermo Scientific™ KingFisher™ Presto as part of an automated purification system with a liquid handler for plate filling in high-throughput laboratories. Utilizing proven KingFisher magnetic particle-based technology, the KingFisher Presto provides high-quality yields of target nucleic acids and proteins for biopharma, biotech and research.

Rapidly perform high content imaging assays with the affordable, compact Thermo Scientific™ CellInsight™ CX5 High Content Screening (HCS) Platform.

The CellInsight CX5 High Content Screening (HCS) Platform is a fast, simple-to-operate, automated cellular imaging and analysis benchtop platform designed for quantitative imaging. For the researcher looking to gain the advantage of quantitative cell analysis for the first time or for those who are looking to increase scale and capacity, the CellInsight CX5 HCS Platform offers a cost effective, but powerful way to quickly automate quantitative cellular imaging.

The Thermo Scientific™ CellInsight™ CX7 High Content Analysis (HCA) Platform is a fast, automated cellular imaging and analysis platform designed for quantitative microscopy.

The CellInsight CX7 HCA Platform offers an integrated way to develop and automate high content assays using a combination of 5-color brightfield and 7-color fluorescence imaging in confocal or wide-field modes.

See how the CellInsight CX7 platform compares to other high content analysis platforms from Thermo Fisher Scientific >>

The Thermo Scientific™ CellInsight™ CX7 LZR High Content Analysis (HCA) Platform is a fast, laser-based, automated cellular imaging and analysis platform designed for quantitative microscopy. This particular configuration includes a CellInsight CX7 LZR Reader, HCS Studio Cell Analysis Software, and Store Image and Database Management Software to visualize and share high content data and images.

The CellInsight CX7 LZR HCA Platform offers a more powerful and integrated way to develop and automate high content assays using a combination of 5-color bright-field and 7-color fluorescence imaging in confocal or wide-field modes. With laser illumination, the Cell Insight LZR HCA Platform enables researchers to image faster in both wide-field and confocal mode.

Featuring excellent optical performance, the Thermo Scientific™ Fluoroskan Microplate Fluorometer is ideal for life science research applications such as fluorometric protein and enzyme studies, molecular interaction studies, nucleic acid quantification, as well as reporter gene, fluorometric kinase, immunological, and cell-based assays.

• High sensitivity for both top and bottom reading
• Onboard dispensers for precise initiation of kinetic reactions
• Powerful and easy-to-use Thermo Scientific™ SkanIt software

Featuring excellent optical performance, the Thermo Scientific™ Fluoroskan FL Microplate Fluorometer and Luminometer is ideal for life science fluorometric and luminometric assays. The Fluoroskan FL fluorometer and luminometer offers plate format versatility, fast plate reading speeds, and up to three dispensers.

• High sensitivity for both top and bottom reading
• Fast reading speed essential for kinetic applications
• Onboard dispensers for precise initiation of kinetic reactions
• Powerful and easy-to-use Thermo Scientific™ SkanIt software

Useful for a variety of luminometric research applications, the Thermo Scientific™ Luminoskan Microplate Luminometer is a compact and robust instrument offering plate format versatility, fast reading speeds, and up to three dispensers. This instrument has passed validation at Promega Corporation to be certified as SkanIt software is quick and easy to learn. It offers easy data analysis (with a comprehensive selection of built-in calculations, including blank subtraction, standard curve creation, classification, quality control, kinetic analysis) and exporting capabilities. Each user can install SkanIt software on his/her own computer to set up assays and analyze data.

Multiskan FC microplate photometer is a reliable instrument for a wide variety of research and routine applications. It reads 96- and 384-well plates and is equipped with shaking and incubation capabilities for temperature critical assays. It can be used as a stand-alone instrument or under PC control with intuitive Thermo Scientific™ SkanIt™ software.

Thermo Scientific™ Multiskan SkyHigh Microplate Spectrophotometer is an easy to use instrument for photometric and turbidimetric applications, especially for DNA, RNA and protein analyses, including endpoint, kinetic, and spectral assays. Results are shown on Multiskan SkyHigh models with a large high-resolution touch screen. SkanIt™ Software is a powerful tool to address the challenging applications.

Designed for bioscience researchers with a wide variety of needs, the Thermo Scientific™ Varioskan™ LUX multimode microplate reader comes equipped with a flexible range of measurement technologies including Absorbance, Fluorescence Intensity, Luminescence, AlphaScreen, and Time-Resolved Fluorescence. The instrument simplifies measurement setup with the automatic dynamic range selection, and its smart safety controls ease workflow and help avoid experimental errors. Varioskan LUX raises the bar for reliability and ease, with technology that works in researchers’ best interest.

The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation.

Wash 96-well plates securely with the Thermo Scientific™ Wellwash™ Microplate Washer, an easy-to-use, reliable microplate-strip washer for routine ELISA applications. The Wellwash features an automated washing, aspiration, dispensing and shaking of 96-well plates and strips in landscape or portrait plate orientation.

Simplify your ELISA workflow by using the eGuide to see our complete portfolio for all your ELISA needs.

Thermo Scientific™ Wellwash™ Versa Microplate Washer, an easy-to-use microplate-strip washer for automated washing, aspiration, dispensing, and shaking of both 96- or 384-well plates for routine ELISA applications.. Designed for high performance and versatility, enjoy the ease of use and convenience provided through a graphical interface, local language version and USB port.

Simplify your ELISA workflow by using the eGuide to see our complete portfolio for all your ELISA needs.

Meet your lab’s specific needs with outstanding flexibility and performance with the easy-to-use Thermo Scientific™ Multidrop™ Combi Reagent Dispenser, designed to support the varied requirements of pharmaceutical and biotechnology laboratories. It combines the most versatile features for reagent dispensing with excellent performance for drug discovery or genomic and proteomic assays.

The Multidrop Combi Reagent Dispenser handles a wide selection of plates and volume range and provides fast dispensing and high-throughput operation via onboard interface or FILLit PC software..

The Thermo Scientific™ Multidrop™ Combi SMART dispenser includes an advanced built-in tracking system, providing easy, reliable traceability for cassette usage.

Experience accurate, consistent, high throughput dispensing for all laboratories with the Thermo Scientific™ Multidrop™ Combi nL Reagent Dispenser. The Combi nL is a nano- to microvolume bulk reagent dispenser with a volume range of 50nL to 50µL. It expands the range of the market leading Multidrop product family, offering easy entry to precise, accurate and reliable low-volume dispensing in pharmaceutical and biotechnology laboratories.

Thermo Scientific™ Multidrop™ Pico 1 Digital Dispenser offers enhanced dispensing precision with volumes between 11pL to 20μL (8 well Cartridge) and 1nL to 200μL (4 well Cartridge) in any well via PicoIT PC software, eliminating the need for manual dilution while providing significant cost and waste reductions. Designed to enable assay miniaturization, boost productivity and improve accuracy of results makes them ideal for a broad array of low-volume applications such as qPCR, dose response curves, drug screening assays and ELISA.

Thermo Scientific™ Multidrop™ Pico 8 Digital Dispenser offers enhanced dispensing precision with volume as low as 11pL in any well via PicoIT PC software. Provides fast and reliable dispensing performance across a large dynamic range, eliminating slow, wasteful, and error-prone manual serial dilutions and enabling assay miniaturization through low-volume assay component addition, such as qPCR, dose response curves, drug screening assays and ELISA.

Extract total protein from mammalian tissue or cell samples using mild but efficient lysis reagents. These reagents have been optimized and validated with specific tissue types, as well as in primary and cultured mammalian cells. Protein extracts are compatible with most protein assays and typical downstream applications. These reagents offer higher protein yield than other commercially available or homemade formulations. The convenient single formulation reagents typically allow samples to be processed in 5–10 minutes. When lysing primary and cultured cells, mechanical disruption is not required.

Choose a total protein extraction reagent for your experiments here >> (https://www.thermofisher.com/ph/en/home/life-science/protein-biology/protein-purification-isolation/cell-lysis-fractionation/cell-lysis-total-protein-extraction.html)

Detergents are amphipathic molecules containing both a nonpolar “tail” having aliphatic or aromatic character, and a polar “head.” The ionic character of the polar head group forms the basis for broad classification of detergents; they may be ionic (charged, either anionic or cationic), nonionic (uncharged) or zwitterionic (having both positively and negatively charged groups, but with a net charge of zero).

Detergents are frequently used in cell lysis reagent formulation, for protein solubilization procedures, in wash buffers for ELISA, and for other protein research methods.

Thermo Scientific™ Pierce Surfact-Amps detergents are highly purified, precisely diluted (10%) formulations that are ideal for applications or assays that are sensitive to contaminants that are present in unpurified detergents. We test every batch to ensure that your detergents contain <1.0 µeq/mL peroxides and carbonyls and package them under nitrogen, to prevent oxidation during storage.

• Superior quality – lower measurable contaminant levels than other leading vendors
• Accurate – precise 10% detergent solution in ultrapure water
• Easy-to-use – solution is simple to dispense and dilute for use
• Exceptionally pure – less than 1.0 µeq/mL peroxides and carbonyls

Learn more about the product here >> (https://www.thermofisher.com/ph/en/home/life-science/protein-biology/protein-purification-isolation/cell-lysis-fractionation/detergents-protein-solubilization.html)

Protease and phosphatase inhibitors are essential components of most cell lysis and protein extraction procedures. These inhibitors block or inactivate endogenous proteolytic and phospholytic enzymes that are released from subcellular compartments during cell lysis and would otherwise degrade proteins of interest and their activation states.

We offer cocktails, tablets, capsules, and individual purified formulations of protease inhibitors and phosphatase inhibitors designed to protect proteins from degradation during cell lysis and extraction.

Choose a broad spectrum cocktail or individual inhibitor here >> (https://www.thermofisher.com/ph/en/home/life-science/protein-biology/protein-purification-isolation/cell-lysis-fractionation/protease-phosphatase-inhibition.html)

After cell lysis or isolation, lysates often contain contaminants that are not compatible with downstream steps. We offer a variety of specialty devices and resins that simply and efficiently desalt, exchange buffers, and remove detergents from samples. In addition, if the protein concentration is too dilute for further processing or analysis, the sample can be concentrated quickly using centrifugal concentrators.

To address the need for rapid removal of small molecules from protein sample preparations, we have expanded our offering with the Pierce Dye and Biotin Removal Spin Columns and 96-Well Filter Plates. These products facilitate removal of non‑conjugated fluorescent dyes, biotin, unused reducing agents, crosslinkers, and other reaction byproducts with excellent protein recovery.

Choose the right protein clean-up product for your experiments >> (https://www.thermofisher.com/ph/en/home/life-science/protein-biology/protein-purification-isolation/protein-dialysis-desalting-concentration.html)

Find the best tools for your workflow from our broad selection of resins and formats for the purification of proteins and antibodies, or create a customized purification solution by covalent attachment of a ligand to one of our activated supports.

The prepared and activated magnetic beads, magnetic agarose beads, standard and Superflow agarose resins, Applied Biosystem POROS resins, and other chromatography media enable protein purification at nearly any scale: assay, screening, batch, pilot, or process. In addition, we offer a range of accessory products for all aspects of purification, including disposable columns and binding and elution buffers including disposable columns, binding and elution buffers, and endotoxin quantitation and removal products.

Pierce BCA Protein Assay Kit is a two-component, high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified protein samples, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracy.

Compare all available BCA protein assays >>

The Pierce Coomassie (Bradford) Protein Assay Kit is a ready-to-use, stable formulation of the traditional Bradford assay reagent for measurement of total protein concentration compared to a protein standard. The kit includes Coomassie Protein Assay Reagent and a package of Albumin Standard ampules.

The Pierce Coomassie Protein Assay Kit is a ready-to-use formulation of the popular assay reagent originally described by Bradford in 1976. When mixed with a protein solution, the acidic Coomassie-dye reagent changes color from brown to blue in proportion to the amount of protein present in the sample. Protein determinations are made by comparison to the color response of protein assay standards, usually prepared as a series of known dilutions of bovine serum albumin (BSA) or bovine gamma globulin (BGG). The simple procedure is adaptable to nearly any volume scale, including test tubes, cuvettes, and microplates. The protein assay is compatible with most salts, solvents, buffers, thiols, reducing substances, and metal chelating agents encountered in protein samples.

Measure protein concentration using a stable formulation of the original Bradford reagent described in the literature with Thermo Scientific™ Pierce Coomassie Protein Assay Kit.

Compare all available Bradford assays >>

The Pierce Modified Lowry Protein Assay is a stable form of a traditional, two-component, folin phenol- and copper-based reagent system to measure total protein concentration compared to a protein standard.

Features include:

• Popular method – widely cited in protein research literature
• Colorimetric – measure with a standard spectrophotometer or plate reader at 750 nm
• Stability – uses a modified cupric sulfate-tartrate reagent that is stable at room temperature
• Assay range – exhibits good linearity in the range 1 to 1500 µg/mL (tested with BSA protein)

See all available protein assays >>

The Pierce 660-nm Protein Assay is a ready-to-use, detergent- and reducing agent-compatible assay reagent to quickly measure total protein concentration compared to a protein standard. The assay is more linear than Coomassie-based Bradford assays and compatible with higher concentrations of most detergents, reducing agents, and other commonly used reagents.

The Pierce 660nm Protein Assay is a quick, ready-to-use colorimetric method for measuring protein concentration. The assay is more linear than coomassie-based Bradford assays and compatible with higher concentrations of most detergents, reducing agents and other commonly used reagents. The accessory Ionic Detergent Compatibility Reagent (IDCR) provides for even broader detergent compatibility, making the Pierce 660nm Protein Assay the only protein assay that is suitable for samples containing Laemmli SDS sample buffer with bromophenol blue.

Although the Pierce 660nm Protein Assay produces a higher level of protein-to-protein variation (37%) than other assays, such as the BCA Protein Assay, the simpler single-reagent format and broader substance compatibility make the 660nm Assay more convenient for many routine applications. The Pierce 660nm Protein Assay can be performed in either a test tube or microplate format.

The assay is based on a proprietary dye-metal complex that binds to proteins in an acidic medium. Upon binding, the reddish dye-metal complex turns to green, resulting in an absorbance shift that is measurable at wavelengths near 660nm (645 to 670). The dye interacts mainly with basic residues in proteins, such as histidine, arginine and lysine and to a lesser extent tyrosine, tryptophan and phenylalanine.

Thermo Scientific™ Prestained Protein Molecular Weight Marker is a mixture of six blue-stained natural proteins (20 to 120 kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents.

Thermo Scientific™ PageRuler Prestained Protein Ladder is a mixture of 10 blue-, orange-, and green-stained proteins (10 to 180 kDa) or 9 blue-, orange- and green-stained proteins (10 to 250 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

Thermo Scientific™ Spectra Multicolor Broad Range Protein Ladder is a 4-color protein standard containing 10 prestained proteins (10 to 260kDa) for use as size standards in gel electrophoresis and Western blotting.

This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. Four different chromophores (blue, orange, green, pink) are bound to the different component proteins, producing a brightly colored ladder with an easy-to-remember pattern.

Thermo Scientific™ SuperSignal Molecular Weight Protein Ladders (20 to 150 kDa) are a ready-to-use mix of recombinant proteins with IgG-binding sites that provide reliable and proportional band intensities in stained gels and immunoblots developed with chemiluminescent, fluorescent, chromogenic or other detection systems.

The SuperSignal Molecular Weight Protein Ladders and SuperSignal Enhanced Molecular Weight Protein Ladders contain a ready-to-use stabilized mixture of eight recombinant proteins ranging in size from 20 to 150 kDa. These recombinant proteins bind antibodies used in the Western blot through an IgG binding site. The protein markers can then be visualized either using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies. The molecular weight marker mix also contains a special pink tracking dye to monitor the electrophoresis run and membrane transfer without the need for additional colored markers. The standards are compatible with nitrocellulose and PVDF membranes, multiple blocking buffers and many other detection methods.

We offer two versions of the standard. The regular formulation is appropriate for use with most rabbit and other non-mouse polyclonal antibodies. The enhanced standard is formulated specifically for applications requiring mouse monoclonal antibodies and experiments where very dilute antibody concentrations are used.

GelCode Blue Stain Reagent is a ready-to-use protein stain based on colloidal coomassie dye G-250 that provides nanogram-level detection in polyacrylamide gels and for in-blot detection.

Features:

• No methanol-acetic acid destaining required
• Fast, one-step, one-hour staining – ready-to-use stain reagent; band development is visible directly in the staining tray; no background from overnight staining; flexible fixing and washing protocol; no gel shrinkage; stained gel can be dried
• Water wash increases staining sensitivity – optional step provides crystal-clear gel background and makes weakly stained bands more visible

Imperial Protein Stain is a ready-to-use colorimetric stain formulated with coomassie dye R-250 that delivers consistent nanogram-level detection of proteins in polyacrylamide electrophoresis gels or nitrocellulose membranes.

Features:

• Sensitive – detects less than 3 ng protein per band with the enhanced protocol (3 hours)
• Time to stain – ready-to-use reagent detects less than 6 ng protein per band in just 20 minutes
• High contrast – intense purple bands are easier to photograph or scan than typical coomassie blue stains
• Compatibility – compatible with downstream mass spectrometry analysis and protein sequencing
• Additional reagents – water washes only; no acid-fixative or methanol destaining solutions required
• Stable – 1-year room-temperature stability

PageBlue Protein Staining Solution is a sensitive, economical formulation of colloidal coomassie G-250 dye for endpoint staining of proteins in polyacrylamide gels and on PVDF membranes without methanol or acetic acid.

Features:

• Easy-to-use – simply soak the gel in the ready-to-use solution and observe stained bands without destaining
• Staining time – 25 to 40 minute protocol
• Safe – does not contain methanol or acetic acid
• Dynamic range – linear dynamic range of 5 to 500 ng
• Economical – can be reused up to three times

Thermo Scientific™ Pierce Silver Stain Kit is a rapid, ultra-sensitive and versatile silver stain system for protein detection in polyacrylamide gels, yielding consistent and reliable results with many 1D and 2D gel types for both first-time and experienced users.

The Pierce Silver Stain Kit is a metallic silver (Ag) protein stain that yields a remarkably clear and uniform gel background while delivering consistent, high-sensitivity staining results at each and every use. In standard mini gels, proteins are detectable at greater than 0.25ng per band or spot. The protocol has been optimized for flexibility by allowing short or overnight gel fixation and staining steps without affecting staining performance (sensitivity or clarity). A short (one-minute) sensitization step, performed after gel fixation, yields results that are free of the characteristically dark or blotchy backgrounds often seen with homemade or other commercially available silver stains. This feature is beneficial when performing densitometric analysis of silver stained gels.

Thermo Scientific™ Pierce Krypton Fluorescent Protein Stain enables sensitive fluorescent visualization (520 nm excitation; 580 nm emission) of proteins separated by polyacrylamide gel electrophoresis or on transfer membranes using popular commercial fluorescence imagers.

This fluorescent protein gel stain is for bright yellow-orange fluorescent detection of proteins separated by 1D or 2D polyacrylamide gel electrophoresis (SDS-PAGE). Krypton Stain uses a protein-binding dye with fluorescence properties (Ex/Em = 520/580 nm) suitable for visualization with a variety of standard laser-based and filter-based fluorescence imagers and documentation systems. Proteins are detectable at concentrations greater than 0.25 ng per band in a typical mini-gel lane (greater than 2 ng for the rapid staining protocol). Krypton Stain provides high signal intensity, exhibits minimal protein-to-protein variation, has an excellent quantitative dynamic range and is compatible with subsequent destaining and analysis by mass spectrometry.

As with other components in a western blotting system, there are many chemiluminescent western blotting substrate choices available. The appropriate selection of a substrate depends on the detection level (sensitivity) required, the target protein’s abundance, the sample abundance and antibody availability. Use the tables below to find the right horseradish peroxidase (HRP) or alkaline phosphatase (AP) substrate for your experimental needs.

Find the right chemiluminescent substrate >>

Unlike chemiluminescent or fluorescent blotting applications, chromogenic substrates do not require special equipment for visualization of the assay results. We offer several chromogenic substrates for your different experimental needs.

Fluorescent western blotting provides accurate, quantitative, stable signals, and the ability to clearly evaluate multiple protein targets on a single blot. We offer optimized reagents, buffers and fluorescent conjugated antibodies so you can get the most from your fluorescent western blots.

Get more from your multiplex westerns >>

Fluorescent secondary antibodies >>

We offer specialized kits for the detection of histidine-tagged proteins, post-translational modifications, multiple target proteins on a single western blot, and target proteins to verify siRNA reagent gene knockdown.

Pierce Fast Western Blotting Kits offer optimized reagents that shorten the time to perform a typical western blot from 4h down to approximately 55 mins. These kits help reduce hands-on and overall blotting time using Thermo Scientific™ ECL and SuperSignal Chemiluminescent substrates.

Each kit includes all reagents necessary to complete a western blot being probed with a mouse or rabbit primary antibody. The protocol requires minimal hands-on time and yields results comparable to classic western blotting with ECL.

Learn more about the Pierce Fast Western Blot Kits

We offer streptavidin, neutravidin and avidin protein conjugates for biotinylated antibody or protein probe detection including HRP, AP conjugates and high-intensity fluorescent and infrared conjugates. Avidin, streptavidin, and neutravidin bind very strongly and specifically to biotin. However, each protein has its limitations in certain assays. Avidin is glycosylated which may lead to nonspecific lectin binding in assays. Streptavidin contains an RYD motif, a bacterial recognition sequence, which can cause background binding with certain samples. Another alternative is to use neutravidin protein which is an exclusive, deglycosylated form of Avidin that avoids the drawbacks of both native avidin and streptavidin.

For a complete list of enzyme-labeled Streptavidin and Avidin conjugates click here

For a complete list of fluorescent streptavidin conjugates click here

Find a variety of colorimetric (chromogenic) substrates for ELISA applications for horseradish peroxidase (HRP) and for alkaline phosphatase (AP) to meet your sensitivity and experimental needs.

Colorimetric ELISA substrates at a glance

TMB (3,3′,5,5′-tetramethylbenzidine) soluble substrates yield a blue color when detecting HRP. The major absorbance maxima or the reaction product are 370 nm and 652 nm. The color then changes to yellow with the addition of sulfuric or phosphoric acid (stop solution) with maximum absorbance at 450 nm. TMB is very sensitive and may produce significant background signal if too much protein or antibody is used. TMB is more quickly oxidized than other HRP substrates, resulting in faster color development.

ABTS (2,2′-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt) is used to detect HRP and yields a water-soluble green end reaction product. The green product has two major absorbance peaks – 410 nm and 650 nm. ABTS is less sensitive than the OPD and TMB substrates for HRP detection. Color development is slow (approximately 20 minutes) which may be advantageous if unacceptable background results from the use of the OPD or TMB substrates due to higher sensitivities. ABTS is available in either tablet or a ready-to-use for formulation.

OPD (o-phenylenediamine dihydrochloride) is used to detect HRP and yields a water soluble yellow-orange reaction product. The reaction product has an absorbance maximum of 492 nm. OPD is available in either powder or tablet forms and easily prepared by dissolving in Stable Peroxide Substrate Buffer or buffered hydrogen peroxide solution.

PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. PNPP produces a yellow water-soluble reaction product that absorbs light at 405 nm. PNPP is available either as a crystalline powder, 5 mg tablets, or as a ready-to-use formulation.

Chemiluminescent ELISA substrates at a glance

SuperSignal ELISA Pico Chemiluminescent Substrate provides excellent performance for a large range of target protein amounts and is easily optimized to detect with greater sensitivity than entry-level colorimetric substrates. Rapid signal generation with 5–30 minute signal stability depending on HRP concentration.

SuperSignal ELISA Femto Maximum Sensitivity Substrate is one of the most sensitive substrates available for ELISA applications. When properly optimized, the lower detection limit is 1 to 10 orders of magnitude lower than commonly used colorimetric substrates. However, without proper optimization, it is easy to overwhelm the system with protein and enzyme, resulting in high background and possibly negative results.

Fluorescent ELISA substrates at a glance

QuantaBlu Fluorogenic Substrate has a large linear detection range with low-end linearity for detection of HRP. The stable fluorescent reaction product has an Emax/Amax of 420 nm/325 nm allowing stopped, non-stopped, and kinetic assays to be performed; an advantage over the more sensitive chemiluminescent substrates.

QuantaRed Enhanced Chemifluorescent Substrate is the most sensitive fluorescent ELISA substrate available for HRP detection. The fluorescent reaction product (resorufin) is stable for 4 hours with an Emax/Amax of 585 nm/570 nm when the reaction is stopped. The red-shifted resorufin reaction product permits detection at a wavelength with less interference from autofluorescence that can occur in biological samples.

The Thermo Scientific™ Pierce Chromogenic Endotoxin Quant Kit is a highly sensitive endpoint assay that accurately measures and detects endotoxin (lipopolysaccharide) in a protein, peptide, nucleic acid, or antibody sample using the amebocyte lysate assay. The kit enables detection within two linear sensitivity ranges of 0.01–0.1 EU/mL and 0.1–1.0 EU/mL.

Features and benefits include:

• Highly sensitive with a broad range – detect as little as 0.01 EU/mL to 1 EU/mL
• Specific – no interference from ß-glucans and suitable for wide range of samples, including protein, vaccine, plasmid, DNA, RNA
• Fast – perform assay in as little as 20 minutes
• End-point chromogenic assay – measure with a standard spectrophotometer or plate reader at 405–410 nm

The Thermo Scientific™ Pierce LAL Chromogenic Endotoxin Quantitation Kit measures the amount of endotoxin in a protein, peptide or antibody sample using the Limulus Amebocyte Lysate (LAL) assay.

The endotoxin concentration in a sample is measured using the Pierce LAL Chromogenic Endotoxin Quantitation Kit via a chromogenic signal generated in the presence of endotoxins. Samples can be measured on a microplate absorbance reader at 405 nm. A standard curve is created using the E. coli endotoxin standard included with each kit to calculate endotoxin levels as low as 0.1 EU/mL. Protein and antibody samples can be assayed in as little as 30 minutes. Determining endotoxin levels is important to assess the efficiency of endotoxin removal methods and prevent endotoxic shock, inflammation and/or sepsis in tissue culture cells and animals injected with endotoxin-contaminated proteins.

Thermo Scientific™ Pierce Glycoprotein Carbohydrate Estimation Kit enables the amount of protein glycosylation to be measured as the percent of total purified protein mass.

The Glycoprotein Carbohydrate Estimation Kit includes six purified glycoprotein standards and the required assay reagents to effectively estimate the amount of oxidizable glycosylation (% carbohydrate by weight) of purified protein samples. Sugar groups in the glycoprotein sample are first oxidized with sodium meta-periodate to produce detectable aldehyde groups. Then the sample is reacted with the colorimetric Glycoprotein Detection Reagent. Absorbance at 550 nm of the resulting purple reaction product is measured with a spectrophotometer or plate reader. Finally, the carbohydrate content in the glycoprotein is calculated by comparison to results from the five glycoprotein standards that are included in the kit.

The EasyPep Mini MS Sample Prep Kit enables efficient and reproducible processing of cultured mammalian cells and tissues for proteomic MS analysis. The kit contains pre-formulated buffers, MS-grade enzyme mix, peptide clean-up columns, and an optimized protocol to generate MS-compatible peptide samples in less than three hours. The kit is optimized to process protein samples from 10–100 µg with a high yield of MS-ready peptides.

Stable isotope labeling using amino acids in cell culture (SILAC) is a powerful method to identify and quantify relative differential changes in complex protein samples. The SILAC method uses in vivo metabolic incorporation of “heavy” 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) analysis for accelerated comprehensive identification, characterization and quantitation of proteins. NeuCode amino acids enable up to four samples to be multiplexed simultaneously.

The Thermo Scientific™ Tandem Mass Tag Reagents are designed to enable identification and quantitation of proteins in different samples using tandem mass spectrometry (MS). All mass tagging reagents within a set have the same nominal mass (i.e., are isobaric) and chemical structure composed of an amine-reactive NHS ester group, a spacer arm (mass normalizer), and a mass reporter.

The standard tandem mass tag (TMT) labeling kits and reagents enable multiplex (6-plex to 11-plex) relative quantitation using high-resolution MS for samples prepared from cells or tissues. The TMT 10plex and 11plex label reagents share an identical structure with TMTzero, TMTduplex, and TMT 6plex reagents but contain different numbers and combinations of 13C and 15N isotopes in the mass reporter.

Check out Thermo Scientific™ TMTpro 16plex label reagents – the next generation of tandem mass tags designed to increase the level of sample multiplexing without compromising on protein identification and quantitation. The new TMTpro 16plex label reagents have the same labeling efficiency and peptide/protein ID rates and provide the same level of quantitative precision as the original TMT reagents, with the benefit of improved quantitative accuracy with larger sample sets.

Depletion is used to reduce the complexity of biological samples such as serum, plasma, or biofluids, which contain high concentrations of albumin and immunoglobulins. These products utilize immunoaffinity techniques to enable the removal of the most abundant proteins, thus enhancing the detection of lower-abundance proteins in both discovery and targeted proteomic analyses.

• Efficient – kits remove >95% of albumin, IgG and up to 12 other abundant proteins
• Fast – spin column formats enable the processing of samples in 5 – 10 minutes
• Economical – cost-effective spin columns are priced for single use; bulk resins available for customized options
• Consistent – one-time use helps prevent abundant protein carryover and experimental variability

The Pierce Quantitative Colorimetric Peptide Assay provides modified BCA reagents for the reduction of Cu+2 to Cu+1 and a proprietary chelator that have been optimized for the quantitation of peptide mixtures. In this reaction, the copper is first reduced by the amide backbone of peptides under alkaline conditions (Biuret reaction). The proprietary chelator then couples with the reduced copper to form a bright red complex with absorbance of 480nm. The signal produced from this reaction is 3-4 fold more sensitive than the Micro-BCA Protein Assay for peptide analysis.

The kit also contains a high quality peptide digest reference standard for use in generating linear standard curves and to serve as a calibration control. This colorimetric peptide assay requires a small amount of sample (20μL) and has a working peptide concentration range of 25 to 1000μg/mL. The increased sensitivity, low sample assay volume, and included reference standard are essential for accurate and robust measurement of peptide digest samples, especially for mass spectrometry applications.

Sequence specific proteases are utilized to cleave proteins into smaller fragments, or peptides. Trypsin is the protease of choice for protein digestion. However, separate or sequential digestion with alternative proteases, such as Glu-C, LysN, Lys-C, Asp-N or Chymotrypsin, can improve individual protein sequence coverage or generate unique peptide sequences for different MS applications. The Thermo Scientific™ Pierce Proteases are highly purified and modified for optimal protein digestion and validated for use in MS. The Thermo Scientific EasyPep Mini MS Sample Prep Kit provides pre-formulated reagents and a robust method for the preparation of high quality samples that are ready for MS analysis in less than 3 hours.

• Comprehensive – broad range of proteases to accommodate a variety of digestion strategies
• Convenient – complete in-gel and in-solution kits are available as well as standalone proteases in multiple pack sizes
• Economical – bulk and custom packaging of some proteases provide more cost effective or optimized options
• Validated – all proteases have been fully tested and processed samples have been analyzed using Thermo Scientific Mass Spectrometers

Post digestion, peptide enrichment and/or fractionation is often necessary to detect peptides from low-abundant proteins and/or for reducing sample complexity for proteomic analysis.

There are several strategies that can be deployed to increase target protein identifications: utilize post-translational modification (PTM) specific affinity binding, such as phosphopeptide enrichment, or through fractionation, such as high pH reversed-phase chromatography which separates peptides by hydrophobicity.

The novel Thermo Scientific™ ActivX™ probes and enrichment kits enable the specific targeting of several enzyme classes, including kinases, GTPases, and serine hydrolases, which are covalently labeled in the active site at the protein level and enriched after digestion.

• Comprehensive – reagents and kits for multiple peptide enrichment and/or fractionation strategies
• Optimized – all kits are designed to maximize protein yield and increase target protein I.D.
• Convenient – easy-to-use spin columns, tips or magnetic formats enable more rapid processing of peptide samples
• Flexible – kits can be used with either cultured mammalian cells or tissues
• Validated – all products have been fully tested and processed samples have been analyzed using Thermo Scientific Mass Spectrometers

After isolation of peptides, salts and buffers can be removed using reversed phase (RP) resins, of which the C18 matrix is the most ideal for the capture of hydrophobic peptides. The peptides bind to reverse-phase columns in high-aqueous mobile phase, salts and buffers are washed off, and the peptides are eluted using a high-organic mobile phase. However, hydophilic peptides, including phosphopeptides, may bind to C18 resins poorly, so graphite spin columns are recommended for better peptide recovery. However, C18 and graphite resins do not efficiently remove detergents, which can contaminate instruments and interfere with column binding, elution and ionization. The Thermo Scientific™ Detergent Removal products efficiently bind to and remove high concentrations of a wide variety of detergents that are commonly used during sample processing.

• Comprehensive – resins and kits for multiple peptide clean-up strategies
• Optimized – all products are designed to maximize peptide yield and LC-MS compatibility
• Convenient – easy-to-use spin tip and columns enable more rapid clean-up of peptides
• Flexible – devices can be used with either cultured mammalian cells or tissues
• Validated – all products have been fully tested and processed samples have been analyzed using Thermo Scientific™ Mass Spectrometers

Thermo Scientific™ Pierce Calibration Solutions for mass spectrometry are ready-to-use liquid formulations that can quickly calibrate Thermo Scientific LC-MS instrumentation. These mixtures are composed of highly purified, ionizable molecules or polymers specifically designed for positive or negative calibration of Thermo Scientific ion trap, orbitrap and triple quadrupole instruments. The calibration solutions are manufactured at an ISO 9001 facility and each lot is quality controlled with strict specifications. The stable solutions are provided in a leak-proof, high-purity PTFE bottle.

• Strong peaks – mixture of instrument-optimized components in a mass spec compatible solvent solution
• Ready to use – load calibration solution into a syringe and inject into the instrument
• High-purity – mass spectrometry-grade reagents in a non-leachable container
• Stable – store at room temperature or refrigerator for more than one year
• Validated – all calibration solutions have been fully tested using Thermo Scientific Mass Spectrometers

The Thermo Scientific™ Pierce Standards are designed for a variety of applications, including sensitivity assessment, determination of digestion efficiency, chromatography assessment and/or as controls for sample analysis. Standards range from single component formulations to complex human-based digests. Each standard is provided with a Certificate of Analysis.

• More choices – different standards to meet a variety of applications/needs
• Stable – store for more than one year at the recommended temperature
• Validated – all standards manufactured in an ISO 9001 facility measured and fully tested using Thermo Scientific Mass Spectrometers

The most commonly used solvents or solvent blends include LC-MS grade water and acetonitrile, with ion pairing agents such as trifluoroacetic acid (TFA), formic acid (FA) or heptafluorobuteric acid (HFBA). These solvents are available as single component formulations or convenient blends. Solvents are manufactured in an ISO9001 facility and are provided with a Certificate of Analysis. Solvents and blends have been validated using Thermo Scientific™ Mass Spectrometers.

• High purity – processed, filtered and sealed to help extend LC-MS column life
• More choices – solvents and solvent blends to meet a variety of applications/needs
• Stable – store for more than one year at room temperature
• Validated – products evaluated with over 20 specifications measured and fully tested using Thermo Scientific Mass™ Spectrometers

Enjoy the enhanced volume adjustment on Thermo Scientific™ Finnpipette™ F1 Variable Volume Pipettes, which is now silent and effortless with textured adjustment knob for a firmer feel when setting volumes. The adjustable finger rest and lightweight design maximize comfort without sacrificing performance

Meet the challenges of highly demanding applications where robustness and durability are essential with the sleek, rugged design of Thermo Scientific™ Finnpipette™ F2 Variable Volume Pipettes. Built with tough PVDF components that stand up to harsh chemicals and the damaging effects of UV light, these variable volume pipettes are fully autoclavable without disassembling. They feature a wide selection of expressly designed tips for optimal performance, precision and accuracy.

Support good laboratory pipetting in your lab with the Thermo Scientific™ F1-ClipTip™ GLP Kits, which offer a variety of single channel pipettes or a mix of single channels and a multichannel pipette packaged with compatible ClipTip tips, and a convenient pipette stand. Select the kit that is right for you, and take advantage of the F1-ClipTip performance and lightweight design to maximize your comfort.

The Thermo Scientific™ E1-ClipTip™ pipette combines interlocking technology which ensures secure tip attachment with the efficiency of electronic pipetting action. E1-ClipTip pipettes are designed for enhanced ergonomic comfort, electronic control, and software-controlled pipetting. It comes equipped with a roster of software tools that allow users to work more easily and quickly. The software includes functionality for multiple profiles and environments. Thermo Fisher’s innovative ClipTip technology improves accuracy and precision. Increase productivity with an intuitive personalized user interface that allows for simple or complex use depending on the application.

An ideal choice for sample transfers ‒ reduce repetition and save time between labware formats with adjustable tip spacing in Equalizer models.

Perform sample transfers between virtually any tube, rack, microplate or horizontal gel box quickly and efficiently with the Thermo Scientific™ E1-ClipTip™ Equalizer Pipette. Adjustable tip spacing allows you to set the distance between tips by simply sliding the scale to expand or contract to the desired labware format. Feel the difference.

Finnpipette Novus delivers performance with greater ease-of-use and reduced risk of injury. Unique to Novus, the adjustable index-finger trigger action prevents common repetitive stress injuries caused by thumb-driven pipettes, and the easy-to-follow prompts guide Novus users through 10 pipetting functions.

Tips that fit any pipette in your lab

Thermo Scientific™ ART tips have been a trusted tool in the lab for decades. Only ART tips contain a self-sealing barrier that seals in aerosols and liquids – eliminating contamination of pipettes and sample cross-contamination during pipetting. ART tips are available in specialty and non-filtered varieties, making it easier than ever to find a tip for every applications and virtually any brand of pipette in your lab. ART delivers the quality, performance and reliability you expect.

These tips are engineered for a secure seal you can feel with your Finnpipettes, creating a pipetting system that delivers optimal accuracy and precision.

Finntip Flex tips are designed with a softer, flexible collar for an ergonomic fit with your Finnpipette to maximize performance and comfort, with reduced attachment and ejection forces to minimize hand strain.

Achieve newfound confidence knowing that once attached, your tips are locked firmly in place, and will not loosen or fall off regardless of application pressure. Thermo ScientificTM ClipTipTM Pipette Tips provide a secure seal for every sample with a unique and innovative interlocking technology that ensures a complete seal on every channel with minimal tip attachment and ejection force. The innovative three interlocking clip design ensures the tip is held securely on the F1- and E1-ClipTipTM pipette systems until – and only until – it is released.

Key Features:

• ClipTip pipette tips feature ‘clips’ that lock tips in place and forms a complete seal
• Each time ClipTip pipette tips are on, they are sealed and will not loosen regardless of application pressure
• ClipTip tip is clipped securely on the F1-ClipTip pipette with a light touch by eliminating the frustration of banging tips on your pipette

Pipette samples with the accuracy and precision you expect from your pipette, every time with Thermo Scientific™ SoftFit-L™ pipette tips. For use with Rainin™ LTS pipettes.

The SoftFit-L Reload System is a complete product offering of pipette tips compatible with LTS™ pipettors made by Rainin Instrument, LLC. Designed in response to customer demands, this unique system provides preloaded pipet tips in color-coded inserts by volume size with the option of an easy-to-use “push down” reload pack. Choose sterile or nonsterile tips, in five volume sizes, with or without our proprietary low-retention technology.

• Reload towers are an environmentally-friendly packaging option that reduces plastic waste and necessary storage space
• Compatible with SoftFit-L empty racks
• Color-coded inserts allows quick identification of tip volume

QSP pipette tips and tubes are made from 100% virgin polypropylene utilizing state of the art molds and molding methods. These products are precision molded for an airtight fit without using a mold release agent in the manufacturing process.

Thin Wall MicroPoint Pipette Tips

Improve small volume pipetting by reducing the liquid retained within the tip while increasing accuracy.

Extra Long Pipette Tips

Ensure maximum sampling with the extended length to reach te bottom of sample vessels and help prevent cross contamination.

Packaging Options

• Bagged
• Individually wrapped
• 96 Tip Rack
• 96 Tip Hinged Rack
• 100 Tip Rack
• 100 Tip Hinged Rack
• 96 Tip Reload System
• 100 Tip Stack Rack System
• 200 Tip Stack Rack System
• 204 Tip Rack

Prepare, transfer and store samples securely

Whether you’re preparing, transferring or storing samples, these multipurpose microcentrifuge tubes and caps can be used with confidence. Choose from a selection of tubes and caps that will suit your application.

Microcentrifuge Tube Selection Tool

Find the Microcentrifuge Tube for your application. Select the tool to view and compare all available tubes.

View selection tool

Select the rack to suit your storage needs

With bench space at a premium, we offer a variety of rack solutions to organize your laboratory work space while conducting, transporting and storing your experiments.

Thermo Scientific™ Forma racks are an essential piece of a complete solution for all of your cold storage needs. Our racks and accessories offer proven reliability and quality you can depend on year after year. Our exceptionally efficient storage configurations maximize your productivity while reducing operating costs. We allow you to maintain sample integrity by reducing the time it takes to access your samples. By reducing risk of exposure to ambient air with quick sample access, your research is safe.

Your choice for optimal scale-up

Find Thermo Scientific™ cell culture flasks designed for consistency, cell health and reproducibility, with the surface and ancillary options you’re looking for in a tissue culture flask. Choose a cell culture flask from a variety of sizes, with culture areas ranging from to and a selection of surface treated offerings to address your specific applications and cell types.

Cell culture dishes in wide range of sizes, styles, and surfaces

We offer cell culture dishes in wide selections of format, material, and surface modification. Each is designed and produced with highest quality standards to promote healthy cells and reproducible results.

Promote healthy, reproducible cell growth with a wide range of quality cell culture plates

Choose from a wide range of styles, sizes, and surfaces to suit a variety of cell culture applications. Thermo Scientific™ Nunc cell culture plates and well plates are available in more than 100 combinations of format and surface, designed to help promote healthy cells and reproducible results. Lot- to-lot cell line testing helps to ensure consistency.

Solid plates are fully molded well plates in either clear polystyrene, or in black or white for use in fluorescence or luminescence studies.

While our clear plates have excellent imaging properties for magnification up to 40x, our optical bottom plates with thin polystyrene film or coverglass bottoms are engineered for superior imaging properties to provide low autofluorescence, absence of a halo effect, improved optical clarity, and high signal-to-noise ratios.

The Thermo Scientific™ Nunc Cell Culture Imaging Products offer comprehensive solutions with flexibility and consistency to meet your needs in cell imaging applications, including fluorescence microscopy, phase contrast microscopy, confocal microscopy, live cell imaging, differential interference contrast (DIC) microscopy, and fluorescence in situ hybridization (FISH).

Download the Nunc Cell Culture Imaging brochure

Select size and packaging options to advance your research with complete confidence when using Thermo Scientific™ Nunc™ Serological Pipettes. These accurate, disposable plastic pipettes are plugged and sterilized.

Thermo Scientific™ Nunc 15mL & 50mL Conical Sterile Polypropylene Centrifuge Tubes are premium, high-quality conical tubes that are environmentally friendly, offer the highest cleanliness with a recyclable plastic rack and allow for increased traceability with the largest writing area on the market. Nunc Labware Products are made from high-purity resins and molded using our state-of-the-art processes. Plastic labware is a safer alternative to glass without sacrificing accuracy.